By Yi G., Sun B., Chen D.
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Anal Chem 76: 6434–6439. Livak KJ, Flood SJ, Marmaro J, Giusti W, Deetz K (1995) Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl 4: 357–362. Matsubatra Y, Kerman K, Kobayashi M, Yamamura S, Morita Y, Tamiya E (2005) Microchamber array based DNA quantification and specific sequence detection from a single copy via PCR in nanoliter volumes. Biosens Bioelectron 20: 1482–1490.
Illustration of the design (a) and the principle of operation (b) of the AFM tweezers. The AFM image can be taken using the sensing probe. To capture the samples, the movable probe, which had the thermal expansion actuator, was operated by applying voltage. 4[c]). 4[d]). 4[e]). 4[f]). 4 Schematic of the fabrication process of the AFM tweezers. The fabrication process was as follows: (a) Si3N4 deposition on a SOI wafer, (b) remove the Si3N4 layer using RIE, (c) thermal oxidation of the Si surface, (d) remove Si3N4 and SiO2 in the shape of tweezers, (e) form the slit between the tweezers’ tips and remove the Si3N4 layer using RIE, (f) KOH etching of Si, (g) remove SiO2 and adjust the probe length, (h) remove the substrate using Deep RIE with an Al mask.
Dissection speed was set to 50 nm/s. After switching to the tapping mode again, the dissected DNA could be observed (b). The same probe was used for the imaging and dissection procedures throughout this experiment. increased stepwise by controlling the amplitude reference in the tapping mode. Amplitude reference is a relative indicator of the loading force in a SII™ AFM operation, and is able to be set at any value. This value represents the probe amplitude reduction from the initial amplitude.